Detection of Cross-Reactivity for Atopic Immunoglobulin E against Multiple Allergens
AUTOR(ES)
Chiou, Yee-Hsuan
FONTE
American Society for Microbiology
RESUMO
The existence of specific immunoglobulin E (IgE) allows us to determine the allergens that cause the allergic disease. For the purposes of allergen avoidance and immunotherapy, the measurement of specific IgE is widely applied in clinical laboratories. However, if IgE from the serum of an allergic patient exhibits reactivity to multiple allergens, it would cause a problem. The present study analyzes whether the serum IgE with multiple reactivity is due to the presence of unique IgE against the common epitope shared by different allergens or the presence of multiple IgEs against different epitopes on different allergens. The quantitative-competitive inhibition tests and the immunoblotting were applied to analyze the immunosimilarity among examined allergens. The result shows that the competitive inhibition of IgE binding between shrimp and crab allergens is higher than those between either shrimp and cockroach or between crab and cockroach. Furthermore, the results of immunoblotting are consistent with those of quantitative-competitive inhibition tests. These results allow us to detect the cross-reactivity for atopic IgE against multiple allergens.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=150535Documentos Relacionados
- Antigenic Cross-Reactivity of Staphylococcal Enterotoxins
- Cross-reactivity of polyclonal serum antibodies generated against Cryptosporidium parvum oocysts.
- Molecular basis for serological cross-reactivity between enteroviruses.
- Evaluation of an immunofluorescence assay for specific detection of immunoglobulin G antibodies directed against Helicobacter pylori, and antigenic cross-reactivity between H. pylori and Campylobacter jejuni.
- Cold hemagglutinin cross-reactivity with Mycoplasma pneumoniae.