Detection of Ehrlichia chaffeensis in human tissue by using a species-specific monoclonal antibody.
AUTOR(ES)
Yu, X
RESUMO
A mouse monoclonal antibody (MAb 1A9) was produced and used in detection of Ehrlichia chaffeensis in human tissues including kidney, liver, and lung by using an indirect immunohistologic stain. MAb 1A9 was specific to E. chaffeensis and did not react with other bacteria, including Ehrlichia canis, which is the organism most closely related to E. chaffeensis. It reacted with an epitope present in two surface proteins of E. chaffeensis with molecular masses of 29 and 27 kDa. E. chaffeensis was easily detected in human tissue by immunohistology with MAb 1A9. This study demonstrates that our MAb can provide a specific and simple method for detection of E. chaffeensis in clinical specimens for establishing an etiologic diagnosis of human ehrlichiosis; it may also provide a tool for the investigation of immunopathologic characteristics in infected patients.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=266402Documentos Relacionados
- Identification of a species-specific antigen in Legionella pneumophila by a monoclonal antibody.
- Identification of the tick-borne relapsing fever spirochete Borrelia hermsii by using a species-specific monoclonal antibody.
- Characterization of an immunoreactive species-specific 19-kilodalton outer membrane protein from Helicobacter pylori by using a monoclonal antibody.
- Monoclonal antibody recognizing a species-specific protein from Streptococcus pneumoniae.
- Detection of rotavirus in human stools by using monoclonal antibody.
