Detection of experimental Chlamydia trachomatis eye infection in conjunctival smears and in tissue culture by use of fluorescein-conjugated monoclonal antibody.
AUTOR(ES)
Taylor, H R
RESUMO
The immunofluorescent staining of conjunctival cytology smears with an antichlamydial monoclonal antibody is a new method for the detection of chlamydial eye infection. Using a monkey model of chlamydial eye infection, we compared the monoclonal antibody staining cytology with the established methods of Giemsa-stained cytology and chlamydial culture with cycloheximide-treated McCoy cells. Compared with either culture or monoclonal antibody-stained cytology, Giemsa cytology had a low sensitivity (30 and 24%, respectively) but was highly specific (100% in each case). Compared with culture, monoclonal antibody-stained cytology was highly sensitive (94%) but appeared to lack specificity (70%). However, examination of the changes in identification rates with time suggested that monoclonal antibody-stained cytology may be even more sensitive than culture and detect lower levels of infection. This study showed that monoclonal antibody-stained cytology of conjunctival smears might be a rapid, efficient, and inexpensive method of diagnosing ocular chlamydial infection.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=271336Documentos Relacionados
- Detection of Chlamydia trachomatis inclusions in Mccoy cell cultures with fluorescein-conjugated monoclonal antibodies.
- Evaluation of a direct fluorescein-conjugated monoclonal antibody for detection of cytomegalovirus in centrifugation culture.
- Optimization of a rapid test by using fluorescein-conjugated monoclonal antibodies for detection of Chlamydia trachomatis in clinical specimens.
- Use of FITC-protein A in place of fluorescein-conjugated anti-gammaglobulins for rapid virus diagnosis by immunofluorescence.
- Secondary fluorescent staining of virus antigens by rheumatoid factor and fluorescein-conjugated anti-IgM.