Detection of fos protein during osteogenesis by monoclonal antibodies.

AUTOR(ES)

De Togni, P

RESUMO

We have generated monoclonal antibodies by using a synthetic peptide corresponding to amino acid positions 4 to 17 of the human fos protein. The antibodies detected both v- and c-fos proteins by immunoprecipitation, immunoblotting, and indirect immunofluorescence. The monoclonal antibodies not only identified the fos protein complex with the cellular 39-kilodalton protein, but also recognized the modified forms of the mouse, rat, and human fos proteins. In day-17 rat embryos, nuclear-staining fos protein could be identified in the cartilage by immunohistochemical staining.

Documentos Relacionados

• Detection of enzyme polymorphism by using monoclonal antibodies.
• Detection of lymphocytes in malignant gliomas by monoclonal antibodies.
• Colorectal carcinoma-specific antigen: detection by means of monoclonal antibodies.
• Detection of P-glycoprotein isoforms by gene-specific monoclonal antibodies.
• Detection of enterovirus 70 with monoclonal antibodies.