Detection of mutations in parC in quinolone-resistant clinical isolates of Escherichia coli.
AUTOR(ES)
Vila, J
RESUMO
The gene parC encodes the A subunit of topoisomerase IV of Escherichia coli. Mutations in the parC region analogous to those in the quinolone resistance-determining region of gyrA were investigated in 27 clinical isolates of E. coli for which ciprofloxacin MICs were 0.0007 to 128 micrograms/ml. Of 15 isolates for which ciprofloxacin MICs were > or = 1 microgram/ml, 8 showed a change in the serine residue at position 80 (Ser-80), 4 showed a change in Glu-84, and 3 showed changes in both amino acids. No mutations were detected in 12 clinical isolates for which ciprofloxacin MICs were < or = 0.25 micrograms/ml. These findings suggest that ParC from E. coli may be another target for quinolones and that mutations at residues Ser-80 and Glu-84 may contribute to decreased fluoroquinolone susceptibility.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=163143Documentos Relacionados
- Quinolone-resistant mutants of escherichia coli DNA topoisomerase IV parC gene.
- Identification for mar mutants among quinolone-resistant clinical isolates of Escherichia coli.
- Alterations in the GyrA subunit of DNA gyrase and the ParC subunit of topoisomerase IV in quinolone-resistant clinical isolates of Klebsiella pneumoniae.
- Quinolone-resistant Escherichia coli
- gyrA and gyrB mutations in quinolone-resistant strains of Escherichia coli.
