Detection of Nuclease Activity in Semisolid and Broth Cultures1
AUTOR(ES)
Lachica, R. Victor F.
RESUMO
A method for the detection of deoxyribonuclease activity in semisolid agar cultures was investigated. When cultures were overlaid with an acridine orangedeoxyribonucleate-agar (ADA) mixture, incubated for 1 to 3 hr, and observed under ultraviolet light, clear halos developed around colonies that produced deoxyribonuclease. A variation of the method for use with broth cultures involved impregnation of filter-paper discs with a small portion of the culture and overlaying the discs with the ADA mixture. This alteration has the advantage that the test tube cultures are easily heat-treated prior to assay to determine the heat resistance of the enzyme.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=377938Documentos Relacionados
- THE DETECTION MICROSCOPICALLY OF COLONIES OF SHIGELLA IN STOOL CULTURES1
- Biological Activity of Reducing-End-Derivatized Oligogalacturonides in Tobacco Tissue Cultures1
- Suppressive Activity of Streptomycin on the Growth of Mycobacterium lepraemurium in Macrophage Cultures1
- Photoinhibition of Growth in Acanthamoeba castellanii Cultures1
- Development of Radiation Resistance in Salmonella Cultures1