Detection of Rifampin Resistance in Mycobacterium tuberculosis by Double Gradient-Denaturing Gradient Gel Electrophoresis
AUTOR(ES)
Scarpellini, Paolo
FONTE
American Society for Microbiology
RESUMO
We applied double gradient-denaturing gradient gel electrophoresis (DG-DGGE) for the rapid detection of rifampin (RMP) resistance from rpoB PCR products of Mycobacterium tuberculosis isolates and clinical samples. The results of this method were fully concordant with those of DNA sequencing and susceptibility testing analyses. DG-DGGE is a valid alternative to the other methods of detecting mutations for predicting RMP resistance.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=89519Documentos Relacionados
- Detection of mutations in GC-rich DNA by bisulphite denaturing gradient gel electrophoresis.
- Rapid detection of the highly polymorphic beta globin framework by denaturing gradient gel electrophoresis.
- Improvement of fragment and primer selection for mutation detection by denaturing gradient gel electrophoresis.
- Polymorphism in the APNH gene, detected by denaturing gradient gel electrophoresis
- Detection of human DNA polymorphisms with a simplified denaturing gradient gel electrophoresis technique.