Determinants of persistence and demyelination of the DA strain of Theiler's virus are found only in the VP1 gene.
AUTOR(ES)
Tangy, F
RESUMO
The DA strain of Theiler's virus persists in the central nervous systems of mice and causes chronic inflammation and demyelination. The GDVII strain, on the other hand, causes an acute encephalitis that kills the host in a matter of days. We constructed a series of recombinants between two infectious cDNA clones of the genomes of DA and GDVII viruses. Analysis of the phenotypes of the recombinant viruses yielded the following results. (i) Determinants of persistence and demyelination are found only in the VP1 capsid protein of DA virus. (ii) Whereas the VP1 capsid protein of DA virus is able to fully attenuate the neurovirulence of GDVII virus and to allow the chimeric virus to persist and demyelinate, the VP1 capsid protein of GDVII virus is unable to render DA virus neurovirulent. (iii) The mere attenuation of the neurovirulence of GDVII virus does not allow it to persist and demyelinate.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=239948Documentos Relacionados
- Determinants of Persistence and Demyelination of the DA Strain of Theiler's Virus Are Found Only in the VP1 Gene
- Replacement of Loop II of VP1 of the DA Strain with Loop II of the GDVII Strain of Theiler’s Murine Encephalomyelitis Virus Alters Neurovirulence, Viral Persistence, and Demyelination
- Theiler's virus persistence and demyelination in major histocompatibility complex class II-deficient mice.
- Trypsin-sensitive neutralization site on VP1 of Theiler's murine encephalomyelitis viruses.
- Importance of amino acid 101 within capsid protein VP1 for modulation of Theiler's virus-induced disease.
