Determination of endotoxins in sugar with the Limulus test.

AUTOR(ES)

Haskå, G

RESUMO

The Limulus amebocyte lysate test has been used for determination of pyrogens in sugar of different qualities. All the samples of domestic white sugar and beet raw sugar produced in Sweden during 1976 had a very low content of endotoxins, less than 10 ng/g of sugar. Imported cane raw sugar was, however, highly contaminated. The highest value obtained corresponds to about 100 mg of Escherichia coli endotoxin per g of raw sugar. Such crude sugar cannot, even after refining, be used for medical purposes. Instead, Swedish beet sugar is used as the raw material for production of invert sugar solutions for parenteral administration. The amount of endotoxin in this sugar is less than 1 ng/g.

Documentos Relacionados

• Endotoxins and their Detection with the Limulus Amebocyte Lysate Test
• Statistical determination of endotoxin content in influenza virus vaccine by the limulus amoebocyte lysate test.
• Comparison of direct serial dilution and most-probable-number methods for determining endotoxins in meats by the Limulus amoebocyte lysate test.
• Bacterial Endotoxins. Structure, Biomedical Significance, and Detection with the Limulus Amebocyte Lysate Test
• Detection of free endotoxin in cerebrospinal fluid by the Limulus lysate test.