Determination of eye irritation potential of low-irritant products: comparison of in vitro results with the in vivo draize rabbit test
AUTOR(ES)
Nóbrega, Andréa Martins da, Alves, Eloisa Nunes, Presgrave, Rosaura de Farias, Costa, Rodrigo Netto, Delgado, Isabella Fernandes
FONTE
Brazilian Archives of Biology and Technology
DATA DE PUBLICAÇÃO
2012-06
RESUMO
In an attempt to build the evaluation strategies to assess the human eye irritation, a reassessment of some in vitro tests is necessary, particularly concerning the non-irritants, mild and moderate irritants. Thus, the correlations between results obtained from the Draize test with the in vitro methods HET-CAM (Hens Egg Test-Chorion Allantonic Membrane) and RBC haemolysis assay to assess the ocular irritancy potential of 20 eye drops were examined. Parameters such as accuracy (%) and specificity (%) were determined. All results were correlated with RBC correctly with the results obtained in the Draize test. The HET-CAM presented four false-positive results, showing a tendency of data overestimation. Despite the high specificity provided mainly by the RBC, it would be necessary to test a wider range of products representing all the scales of irritation to confirm its ability to be used as a first alternative to test products that could be presumptive non-irritants.
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