Determination of hippurate hydrolysis by gas-liquid chromatography.
AUTOR(ES)
Wallace, P L
RESUMO
A rapid gas-liquid chromatographic procedure was developed to determine hippurate hydrolysis by microorganisms. Bacterial cells were inoculated into 0.4 ml of 1% sodium hippurate and incubated for 2 h at 37 degrees C. Cells were removed by centrifugation, and the benzoate released by enzyme activity was converted to methyl benzoate and analyzed by gas-liquid chromatography. This procedure is sensitive, and its specificity provides a high degree of reliability for organisms with weak hippuricase activity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=269324Documentos Relacionados
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