Determination of secondary structure in the initiation region of ovalbumin mRNA.
AUTOR(ES)
Liarakos, C D
RESUMO
We have analyzed the secondary structure in the region surrounding the initiation codons of both cellular and synthetic versions of ovalbumin mRNA. RNase V1 cleavage sites and structure-dependent, chemically modified bases in cellular ovalbumin mRNA were determined by reverse transcription of hen poly A(+) RNA using ovalbumin-specific, synthetic DNA primers. These results indicate an extensive region of unpaired nucleotides preceding the initiation codon and a region of base-paired nucleotides including and following the initiation codon. A synthetic ovalbumin mRNA (SP65.OV) was prepared by run-off transcription of a cloned ovalbumin cDNA (pSP65.OV). Identical regions of hen ovalbumin and SP65.OV mRNAs gave identical patterns of structure-dependent base modifications. A computer program for determining RNA secondary structure was used to find a 5'-region structure for ovalbumin mRNA that is consistent with our data.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=339008Documentos Relacionados
- Characterization of the effects on ovalbumin mRNA of aminomethyl-trimethylpsoralen photoreaction with hen oviduct mRNA.
- Influences of mRNA secondary structure on initiation by eukaryotic ribosomes.
- Structure and function of a bacterial mRNA stabilizer: analysis of the 5' untranslated region of ompA mRNA.
- Secondary structure model for the complete simian virus 50 late precursor mRNA.
- The 5'-end structure of ovalbumin mRNA in isolated nuclei and polysomes.
