Determination of serum neutralization antibodies to rabies virus by a modified counterimmunoelectrophoresis test.
AUTOR(ES)
Díaz, A M
RESUMO
A modified counterimmunoelectrophoresis (CIE/SN) technique was developed as a diagnostic procedure for the detection of serum-neutralizing antibodies to rabies virus. CIE/SN test results with sera from individuals undergoing postexposure rabies immunization compared favorably with those obtained by the standard mouse neutralization test procedure. Fractionation of selected sera by Sephadex G-200 column chromatography demonstrated that serological activity involved in both procedures was present in the immunoglobulin G-containing fractions of the sera. The mean advantage of the CIE/SN over the standard mouse neutralization test consists in considerably reducing the time required for results, a factor which can be of clinical importance in evaluating antibody levels to rabies virus. In addition, the CIE/SN method is simple to perform and inexpensive.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=273550Documentos Relacionados
- Comparison between a modified counterimmunoelectrophoresis test and the indirect fluorescent-antibody test for detection of antibodies to rabies virus in human sera.
- Comparison of a modified counterimmunoelectrophoresis test and a microimmunodiffusion test for detection of pseudorabies virus antibodies in porcine sera.
- Measurement of antibodies to varicella-zoster virus by using a latex agglutination test.
- Inactivation of rabies virus in reagents used for the fluorescent rabies antibody test.
- Evaluation of a poliovirus-binding inhibition assay as an alternative to the virus neutralization test.
