Determination of the Chromosomal Location of a Glutamate Oxaloacetate Transaminase Structural Gene Using Triticum-Agropyron Translocations
AUTOR(ES)
Hart, Gary E.
RESUMO
The glutamate oxaloacetate transaminase (GOT) zymogram phenotypes of a series of 15 translocation lines, a chromosome addition line and a chromosome substitution line were determined. In each of the translocation lines a segment of the long arm of Triticum aestivum chromosome 3D has been replaced by a portion of an Agropyron elongatum homoeologue. Evidence was obtained that the products of the T. aestivum GOT-3 triplicate structural gene set randomly dimerize with the product of the homoeologous A. elongatum gene. Each translocation chromosome was found to carry either Got-D3 or Got-Ag3. By correlating the zymogram phenotype expressed by each translocation line with the observed frequency of meiotic pairing of each 3D/3Ag translocation chromosome with telocentric-3DL, it was shown that Got-D3 is located in the proximal portion of 3DL, slightly more than 4.3 crossover units from the centromere. The results of this genetic study confirm and extend earlier conclusions derived from cytogenetic studies as to the physical nature of the various 3D/3Ag chromosomes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1213503Documentos Relacionados
- Glutamate Oxaloacetate Transaminase (Got) Genetics in the Mouse: Polymorphism of Got-1
- Chromosomal Location of Two Mitochondrial Malate Dehydrogenase Structural Genes in ZEA MAYS Using Trisomics and B-A Translocations
- Chromosomal Location of the Structural Gene for Glycerol Kinase in Escherichia coli
- Chromosomal location of a structural gene for the RNA polymerase sigma factor in Escherichia coli.
- Role of Glutamate-oxaloacetate Transaminase and Malate Dehydrogenase in the Regeneration of NAD+ for Glycine Oxidation by Spinach leaf Mitochondria 1
