Determination of the promoter strength of the gene encoding Escherichia coli heat-stable enterotoxin II.
AUTOR(ES)
Spandau, D F
RESUMO
We studied the promoter strength of the gene encoding the Escherichia coli heat-stable enterotoxin II (STII). The promoter region and a portion of the 5' coding sequence of the STII gene were fused to the lacZ gene so that the production of beta-galactosidase was under the control of the STII gene promoter. The strength of the STII gene promoter was compared with that of the ompF and lac operons, which were similarly fused to the lacZ gene. The beta-galactosidase produced by the hybrid genes was assayed in vitro by using cell extracts. The mRNA transcribed by each promoter was assayed by Northern blot analysis and by in vitro transcription. The results suggest that the STII gene is regulated by a relatively weak promoter.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=212008Documentos Relacionados
- Purification and characterization of Escherichia coli heat-stable enterotoxin II.
- Some properties of purified Escherichia coli heat-stable enterotoxin II.
- Disulfide bond formation and secretion of Escherichia coli heat-stable enterotoxin II.
- Enzyme-linked immunosorbent assay for Escherichia coli heat-stable enterotoxin type II.
- Isolation and nucleotide sequence determination of a gene encoding a heat-stable enterotoxin of Escherichia coli.