Determination of the rate of base-pair substitution and insertion mutations in retrovirus replication.

AUTOR(ES)

Dougherty, J P

RESUMO

We recently described a protocol for determination of retrovirus mutation rates, that is, the mutation frequency in a single cycle of retrovirus replication (J.P. Dougherty and H.M. Temin, Mol. Cell. Biol. 6:4378-4395, 1987; J.P. Dougherty and H.M. Temin, p. 18-23, in J. H. Miller and M. P. Calos, ed., Gene Transfer Vectors for Mammalian Cells, 1987). We used this protocol to determine the mutation rates for defined mutations in a replicating retrovirus by using a spleen necrosis virus-based vector. We determined that the mutation rate for a single base pair substitution during replication of this avian retrovirus is 2 x 10(-5) per base pair per replication cycle and the insertion rate is 10(-7) per base pair per replication cycle. It will be possible to use this protocol to determine mutation rates for other retroviruses.

Documentos Relacionados

• The Influence of Neighboring Base Pairs upon Base-Pair Substitution Mutation Rates
• 1H NMR determination of base-pair lifetimes in oligonucleotides containing single base mismatches
• Photochemical selectivity in guanine–cytosine base-pair structures
• A structural snapshot of base-pair opening in DNA
• Two improved promoter sequences for the beta-lactamase expression arising from a single base-pair substitution.