Detoxification of Liquid Cultures of Bordetella pertussis by Forced Aeration at High pH

AUTOR(ES)

Lane, A. G.

RESUMO

Cultures of Bordetella pertussis cultivated in shake flasks were invariably highly toxic for mice, but cultures of the same strain grown in vortex-aerated vessels were nontoxic at the time of harvest. Results reported here indicate that toxin is present during the early log phase in vortex-aerated cultures, but is lost as the cultivation proceeds. The loss of toxicity is apparently due to denaturation of the toxin by the combined influence of vigorous aeration and elevated pH.

Documentos Relacionados

• EFFECT OF AERATION ON pH VALUES OF BACTERIAL CULTURES
• Production of Solvents by Clostridium acetobutylicum Cultures Maintained at Neutral pH
• Lowering of cytoplasmic pH is essential for growth of Streptococcus faecalis at high pH.
• Comparing electrophoresis at alkaline pH and high performance liquid chromatography to diagnose Hb S-like hemoglobin
• Appearance of Mouse-Lethal Toxin in Liquid Cultures of Bordetella pertussis