Development of a diagnostic test for Johne's disease using a DNA hybridization probe.

AUTOR(ES)

Hurley, S S

RESUMO

A DNA probe, M13 mpHAW71, that detects Mycobacterium paratuberculosis in the fecal material of infected animals was developed for use in the diagnosis of Johne's disease. The probe detected as few as 10(5) M. paratuberculosis when hybridized under stringent conditions to total genomic DNA purified from bovine fecal material. When the probe was used diagnostically, it did not differentiate members of the Mycobacterium avium-M. intracellulare-M. paratuberculosis complex. Compared with culturing, the DNA probe identified 34.4% more mycobacterium-containing fecal samples, and testing took only 72 h to complete.

Documentos Relacionados

• Preparation of a specific RNA probe for detection of Mycobacterium paratuberculosis and diagnosis of Johne's disease.
• Dot blot hybridization assay for chicken anemia agent using a cloned DNA probe.
• Evaluation of a commercial enzyme-linked immunosorbent assay for Johne's disease.
• Development of species-specific enzyme-linked immunosorbent assay for diagnosis of Johne's disease in cattle.
• Detection of DNA "fingerprints" of cultivated rice by hybridization with a human minisatellite DNA probe.