Development of a mariner-Based Transposon for Use in Sorangium cellulosum
AUTOR(ES)
Julien, Bryan
FONTE
American Society for Microbiology
RESUMO
In order to generate marked insertions in the myxobacterium Sorangium cellulosum, a transposon based on the eukaryotic mariner transposon was developed. The transposition frequency was increased with the use of a mutated tnp gene. The transposon randomly inserts into the chromosome, as demonstrated by targeted mutagenesis of the epoK gene.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=201241Documentos Relacionados
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