Development of a monoclonal antibody-based immunoassay for the detection and quantification of Anguillospora longissima colonizing leaf material.

AUTOR(ES)

Bermingham, S

RESUMO

Monoclonal antibodies (MAbs) to the aquatic hyphomycete Anguillospora longissima were raised in mice by using a coimmunization program. A cell line was raised that produced a MAb of the immunoglobulin M class that was specific for A. longissima both in an enzyme-linked immunosorbent assay (ELISA) and by immunofluorescence but that did not recognize other members of the aquatic hyphomycetes. This MAb (AL-HH8c) was used to develop a quantitative ELISA in vitro. The antigen recognized by AL-HH8c is produced throughout the mycelium, irrespective of mycelial age and culture conditions. By using this MAb, mycelium of A. longissima colonizing leaf material can be detected by ELISA, immunofluorescence, and immunoenzymatic staining methods.

Documentos Relacionados

• Monoclonal antibody-based enzyme immunoassay for pediocins of Pediococcus acidilactici.
• Monoclonal antibody-based enzyme immunoassay for Giardia lamblia antigen in human stool.
• Evaluation of a commercial monoclonal antibody-based enzyme immunoassay for detection of adenovirus types 40 and 41 in stool specimens.
• Monoclonal antibody-based immunoassay for type A Clostridium botulinum toxin is comparable to the mouse bioassay.
• Detection of West Nile Virus Antigen in Mosquitoes and Avian Tissues by a Monoclonal Antibody-Based Capture Enzyme Immunoassay