Development of a shaker culture of Buffalo green monkey kidney cells: potential use for detection of enteroviruses.
AUTOR(ES)
Goldstein, G
RESUMO
Buffalo green monkey kidney cells were adapted to grow as shaker cultures. Replication of environmental and clinical isolates of poliovirus, coxsackievirus, and echovirus in these cultures was analyzed by plaque assay and compared with replication in Buffalo green monkey kidney cell monolayers and HEp-2 cell shaker cultures. Dose-response tests with various concentrations of Mahoney type 1 poliovirus indicated that Buffalo green monkey kidney cell shaker cultures could detect as little as 1 PFU in an inoculum of 0.2 ml. These data suggest that Buffalo green monkey kidney cell shaker cultures can be effectively used for the detection of small quantities of enteroviruses from environmental sources.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=242012Documentos Relacionados
- Nucleic acid detection systems for enteroviruses.
- Isolation of Chlamydia using McCoy cells and Buffalo green monkey cells.
- Tissue typing amniotic fluid cells: potential use for detection of contaminating maternal cells.
- Comparison of buffalo green monkey cells and McCoy cells for isolation of Chlamydia trachomatis in a microtiter system.
- Nonisotopic oligomeric probes for the human enteroviruses.
