Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro.
AUTOR(ES)
Kawasaki, E S
RESUMO
The Philadelphia chromosome is present in more than 95% of chronic myeloid leukemia patients and 13% of acute lymphocytic leukemia patients. The Philadelphia translocation, t(9;22), fuses the BCR and ABL genes resulting in the expression of leukemia-specific, chimeric BCR-ABL messenger RNAs. To facilitate diagnosis of these leukemias, we have developed a method of amplifying and detecting only the unique mRNA sequences, using an extension of the polymerase chain reaction technique. Diagnosis of chronic myeloid and acute lymphocytic leukemias by this procedure is rapid, much more sensitive than existing protocols, and independent of the presence or absence of an identifiable Philadelphia chromosome.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=281827Documentos Relacionados
- Isolation and Identification of Lymphocytic and Myelogenous Leukemia-Specific Sequences in Genomes of Gibbon Oncornaviruses
- The translocation (6;9), associated with a specific subtype of acute myeloid leukemia, results in the fusion of two genes, dek and can, and the expression of a chimeric, leukemia-specific dek-can mRNA.
- Cell death induction by the acute promyelocytic leukemia-specific PML/RARα fusion protein
- Leukemia-Specific DNA Sequences in Leukocytes of the Leukemic Member of Identical Twins
- Chronic myelogenous leukemia shapes host immunity by selective deletion of high-avidity leukemia-specific T cells