Differential distribution of RNA polymerase B and nonhistone chromosomal proteins in polytene chromosomes of Drosophila melanogaster.
AUTOR(ES)
Kabisch, R
RESUMO
The distribution patterns of nonhistone chromosomal proteins (NHCP) associated with pulse-labeled RNA were determined by indirect immunofluorescence on salivary gland chromosomes of Drosophila melanogaster using monoclonal antibodies. By staining for two different antigens simultaneously, using antibodies tagged with different fluorescent probes, it became possible to position RNA-associated antigens as well as RNA polymerase B in relation to each other. Three separate staining patterns could be observed with anti-NHCP antibodies, none of which showed a pattern which was identical with that of RNA polymerase B. Furthermore, no correlation with the synthesis of the primary trancript, as monitored by the RNA polymerase B content of chromosomal sites, could be found by following the fluorescence patterns during inactivation of intermolt puffs or activation of early ecdysone-induced puffs. Finally, no strict correlation was observed between puffing activity and the accumulation of a certain antigen in these selected chromosomal sites.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=555146Documentos Relacionados
- Localization of RNA polymerase in polytene chromosomes of Drosophila melanogaster.
- Localization of ecdysterone on polytene chromosomes of Drosophila melanogaster.
- Puffing activities and binding of ecdysteroid to polytene chromosomes of Drosophila melanogaster.
- Association of cyclic GMP with gene expression of polytene chromosomes of Drosophila melanogaster.
- Locations of chromosomal proteins in polytene chromosomes.