Differential effect of detergents on the alkaline denaturation of haemoglobin in maternal and fetal blood, with particular reference to Triton X-100.
AUTOR(ES)
Duck-Chong, C G
RESUMO
In this investigation, the alkaline denaturation of haemoglobin in the blood of pregnant women and in cord blood obtained from newborn infants was followed by measuring the increase in absorbance at 375 nm. As expected, in the absence of detergent, the haemoglobin of cord blood was much more resistant to alkaline denaturation than that of maternal blood. However, in the presence of Triton X-100, a non-ionic detergent, the sensitivity of fetal haemoglobin to alkali was comparable to that of adult haemoglobin. Similar results were obtained using the non-ionic detergents, Brij-35, Tween 80 and Nonidet P40, but the anionic detergent, sodium deoxycholate, was apparently without effect. These findings form the basis of a rapid and sensitive method for discriminating between maternal and fetal blood in biological specimens.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=498420Documentos Relacionados
- Cellular lysis of Streptococcus faecalis induced with triton X-100.
- Molecular weight of bacteriorhodopsin solubilized in Triton X-100.
- Effect of zinc ion on the hemolytic activity of thermostable direct hemolysin from Vibrio parahaemolyticus, streptolysin O, and Triton X-100.
- Characterization of a succinate dehydrogenase complex solubilized from the cytoplasmic membrane of Bacillus subtilis with the nonionic detergent Triton X-100.
- Capacity of the outer membrane of a gram-negative marine bacterium in the presence of cations to prevent lysis by Triton X-100.