Differential gene expression profiles of hepatocellular carcinomas associated or not with viral infection
AUTOR(ES)
Bellodi-Privato, M., Kubrusly, M.S., Stefano, J.T., Soares, I.C., Wakamatsu, A., Oliveira, A.C., Alves, V.A.F., Bacchella, T., Machado, M.C.C., D’Albuquerque, L.A.C.
FONTE
Brazilian Journal of Medical and Biological Research
DATA DE PUBLICAÇÃO
06/11/2009
RESUMO
Chronic hepatitis B (HBV) and C (HCV) virus infections are the most important factors associated with hepatocellular carcinoma (HCC), but tumor prognosis remains poor due to the lack of diagnostic biomarkers. In order to identify novel diagnostic markers and therapeutic targets, the gene expression profile associated with viral and non-viral HCC was assessed in 9 tumor samples by oligo-microarrays. The differentially expressed genes were examined using a z-score and KEGG pathway for the search of ontological biological processes. We selected a non-redundant set of 15 genes with the lowest P value for clustering samples into three groups using the non-supervised algorithm k-means. Fisher’s linear discriminant analysis was then applied in an exhaustive search of trios of genes that could be used to build classifiers for class distinction. Different transcriptional levels of genes were identified in HCC of different etiologies and from different HCC samples. When comparing HBV-HCC vs HCV-HCC, HBV-HCC/HCV-HCC vs non-viral (NV)-HCC, HBC-HCC vs NV-HCC, and HCV-HCC vs NV-HCC of the 58 non-redundant differentially expressed genes, only 6 genes (IKBKβ, CREBBP, WNT10B, PRDX6, ITGAV, and IFNAR1) were found to be associated with hepatic carcinogenesis. By combining trios, classifiers could be generated, which correctly classified 100% of the samples. This expression profiling may provide a useful tool for research into the pathophysiology of HCC. A detailed understanding of how these distinct genes are involved in molecular pathways is of fundamental importance to the development of effective HCC chemoprevention and treatment.
Documentos Relacionados
- Insight into hepatocellular carcinogenesis at transcriptome level by comparing gene expression profiles of hepatocellular carcinoma with those of corresponding noncancerous liver
- Gene expression profiling associated with the progression to poorly differentiated thyroid carcinomas
- Mouse Cytokine Profiles Associated with Brucella abortus RB51 Vaccination or B. abortus 2308 Infection
- Chromosomal translocation and inverted duplication associated with integrated hepatitis B virus in hepatocellular carcinomas.
- Differential expression patterns of the hox gene are associated with differential growth of insect hind legs