Differential Persistence among Genomovars of the Burkholderia cepacia Complex in a Murine Model of Pulmonary Infection
AUTOR(ES)
Chu, Karen K.
FONTE
American Society for Microbiology
RESUMO
Cystic fibrosis patients infected with strains from different genomovars of the Burkholderia cepacia complex can experience diverse clinical outcomes. To identify genomovar-specific determinants that might be responsible for these differences, we developed a pulmonary model of infection in BALB/c mice. Mice were rendered leukopenic by administration of cyclophosphamide prior to intranasal challenge with 1.6 × 104 bacteria. Five of six genomovar II strains persisted at stable numbers in the lungs until day 16 with minimal toxicity, whereas zero of seven genomovar III strains persisted but resulted in variable toxicity. We have developed a chronic pulmonary model of B. cepacia infection which reveals differences among genomovars in terms of clinical infection outcome.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=127911Documentos Relacionados
- A Murine Model for Infection with Burkholderia cepacia with Sustained Persistence in the Spleen
- DNA-Based Diagnostic Approaches for Identification of Burkholderia cepacia Complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia Genomovars I and III
- Attenuated Virulence of a Burkholderia cepacia Type III Secretion Mutant in a Murine Model of Infection
- Correlation between an In Vitro Invasion Assay and a Murine Model of Burkholderia cepacia Lung Infection
- Persistence of Burkholderia multivorans within the Pulmonary Macrophage in the Murine Lung
