Differential synthesis of blocked and unblocked 5'-termini in reovirus mRNA: effect of pyrophosphate and pyrophosphatase.
AUTOR(ES)
Furuichi, Y
RESUMO
Viral mRNA synthesized in vitro by reovirusassociated enzymes contained 5'-terminal ppG, GpppG, or m7GpppGm. Selective synthesis of one type of 5'-end was obtained by adjusting the composition of the transcription reaction mixture based on a series of reactions proposed for formation of blocked, methylated 5'-terminal sequences of mRNA (capformation). The presence of inorganic pyrophosphate increased the proportion of ppG 5'-ends, while addition of pyrophosphatase yielded mRNA with 5'-terminal GpppG, which in the presence of a methyl donor was converted to m7GpppGm.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=431132Documentos Relacionados
- Blocked and Unblocked 5′ Termini in Reovirus Genome RNA
- Methylated, blocked 5 termini in HeLa cell mRNA.
- Nucleotide sequences at the 5' termini of reovirus mRNA's.
- Nucleotide sequences and mapping of novel heterogenous 5'-termini of adenovirus 2 early region 4 mRNA.
- Pea cytochrome oxidase subunit II gene has no intron and generates two mRNA transcripts with different 5′-termini