Dinitrogen Fixation by Cultures of Frankia sp CpIl Demonstrated by 15N2 Incorporation 1

AUTOR(ES)

Torrey, John G.

RESUMO

The filamentous bacterium Frankia of the Actinomycetales, isolated from the nitrogen-fixing root nodules of certain woody plants, has shown nitrogenase activity in culture, using the acetylene reduction method. In the present work, nitrogenase activity in pure cultures of Frankia sp. CpIl is confirmed using mass spectrometric measurements of 15N2 incorporation. After addition of carrier NH4+ to digested cultures, those exposed to 15N2 (25 atom%) had a 15N content of 3.16 atom% compared to 0.354 atom% 15N in the controls.

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