Dinucleotide Sequences at the 5′ Ends of Vaccinia Virus mRNA's Synthesized In Vitro
AUTOR(ES)
Keith, Jerry M.
RESUMO
The diversity of dinucleotide sequences at the 5′ ends of vaccinia virus mRNA's was determined by a two-dimensional electrophoresis procedure. RNA labeled with S-adenosyl[methyl-3H]methionine was synthesized in vitro by enzymes present in vaccinia virus cores. The RNA, ending in m7G(5′)pppNmpN−, was β-eliminated and treated with alkaline phosphatase. After digestion with RNases T2, T1, and A, all eight possible dinucleotides containing Gm and Am were identified. They are, in decreasing order of abundance: GmpUp (22%), AmpCp (18%), GmpAp (16%), GmpCp (15%), AmpAp (11%), AmpUp (10%), AmpGp (7%), and GmpGp (2%).
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=353680Documentos Relacionados
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