Direct Measurement of the pK Values of an Alkaline Bohr Group in Human Hemoglobin

AUTOR(ES)

Kilmartin, J. V.

RESUMO

Proton nuclear magnetic resonance spectra of normal and des-(his 146β) human hemoglobin in the aromatic resonance region have been compared at different pH values. From these measurements, a pK value for histidine 146β of 7.1 in carbonmonoxyhemoglobin and 8.0 in deoxyhemoglobin has been found. These pK values confirm the proposed role of histidine 146β in the alkaline Bohr effect.

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