Dissociation of N2 Gas-induced Monomeric Ribosomes and Functioning of the Derived Subunits in Protein Synthesis in Pea 1
AUTOR(ES)
Lin, C. Y.
RESUMO
The dissociation of N2 gas-induced monomeric ribosomes from the pea root was studied by varying the concentration of KCl (or NH4Cl) and MgCl2 in the presence of dithiothreitol. These monoribosomes were shown to dissociate completely into subunits at 0.5m KCl or NH4Cl in the presence of 5 mm MgCl2. The 40S subunits were more susceptible to structural change in KCl than were the 60S subunits. On the other hand, the 60S subunits appeared to be more labile to NH4Cl.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=396904Documentos Relacionados
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