Distinct requirements for C.elegans TAFIIs in early embryonic transcription
AUTOR(ES)
Walker, Amy K.
FONTE
Oxford University Press
RESUMO
TAFIIs are conserved components of the TFIID, TFTC and SAGA-related mRNA transcription complexes. In yeast (y), yTAFII17 is required broadly for transcription, but various other TAFIIs appear to have more specialized functions. It is important to determine how TAFIIs contribute to transcription in metazoans, which have larger and more diverse genomes. We have examined TAFII functions in early Caenorhabditis elegans embryos, which can survive without transcription for several cell generations. We show that taf-10 (yTAFII17) and taf-11 (yTAFII25) are required for a significant fraction of transcription, but apparently are not needed for expression of multiple developmental and other metazoan-specific genes. In contrast, taf-5 (yTAFII48; human TAFII130) seems to be required for essentially all early embryonic mRNA transcription. We conclude that TAF-10 and TAF-11 have modular functions in metazoans, and can be bypassed at many metazoan-specific genes. The broad involvement of TAF-5 in mRNA transcription in vivo suggests a requirement for either TFIID or a TFTC-like complex.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=125634Documentos Relacionados
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