Distribution of two selenonucleosides among the selenium-containing tRNAs from Methanococcus vannielii
AUTOR(ES)
Ching, Wei-Mei
RESUMO
Naturally occurring seleno-tRNAs from Methanococcus vannielii account for 13-20% of the total tRNA population. Two different selenium-modified nucleosides were detected in these seleno-tRNAs. Of the total selenium incorporated, about 60% is present in 5-methylaminomethyl-2-selenouridine, and the other 40% occurs in a second selenonucleoside with spectral characteristics typical of a derivative of 2-selenouridine. The 5-methylaminomethyl-2-selenouridine was found in the seleno-tRNA species present in the early fractions of a linear salt gradient elution profile from a reversed-phase chromatographic system 5(RPC-5) column, whereas the second selenonucleoside occurred in the tRNA species eluted late in the profile.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=344609Documentos Relacionados
- Occurrence of selenium-containing tRNAs in mouse leukemia cells.
- Selenium-containing tRNAs from Clostridium sticklandii: cochromatography of one species with L-prolyl-tRNA.
- Biosynthesis of selenium-modified tRNAs in Methanococcus vannielii.
- Selenium-containing tRNAGlu from Clostridium sticklandii: correlation of aminoacylation with selenium content.
- Stereochemical studies of a selenium-containing hydrogenase from Methanococcus vannielii: determination of the absolute configuration of C-5 chirally labeled dihydro-8-hydroxy-5-deazaflavin cofactor.
