Diversity Arrays: a solid state technology for sequence information independent genotyping
AUTOR(ES)
Jaccoud, Damian
FONTE
Oxford University Press
RESUMO
Here we present the successful application of the microarray technology platform to the analysis of DNA polymorphisms. Using the rice genome as a model, we demonstrate the potential of a high-throughput genome analysis method called Diversity Array Technology, DArT‘. In the format presented here the technology is assaying for the presence (or amount) of a specific DNA fragment in a representation derived from the total genomic DNA of an organism or a population of organisms. Two different approaches are presented: the first involves contrasting two representations on a single array while the second involves contrasting a representation with a reference DNA fragment common to all elements of the array. The Diversity Panels created using this method allow genetic fingerprinting of any organism or group of organisms belonging to the gene pool from which the panel was developed. Diversity Arrays enable rapid and economical application of a highly parallel, solid-state genotyping technology to any genome or complex genomic mixtures.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=29632Documentos Relacionados
- Persistence of Tandem Arrays: Implications for Satellite and Simple-Sequence Dnas
- Diversity Arrays Technology (DArT) for whole-genome profiling of barley
- FMR1 triplet arrays: paying the price for perfection
- Extracting biological information from DNA arrays: an unexpected link between arginine and methionine metabolism in Bacillus subtilis
- Genetic diversity of carotenoid-rich bananas evaluated by Diversity Arrays Technology (DArT)