DNA binding by the Xis protein of the conjugative transposon Tn916.
AUTOR(ES)
Rudy, C K
RESUMO
We purified the Xis protein of the conjugative transposon Tn916 and showed by nuclease protection experiments that Xis bound specifically to sites close to each end of Tn916. These specific binding sites are close to, and in the same relative orientation to, binding sites for the N-terminal domain of Tn916 integrase protein. These results suggest that Xis is involved in the formation of nucleoprotein structures at the ends of Tn916 that help to correctly align the ends so that excision can occur.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=179005Documentos Relacionados
- Excision of a conjugative transposon in vitro by the Int and Xis proteins of Tn916.
- Specific DNA cleavage mediated by the integrase of conjugative transposon Tn916.
- Xis Protein Binding to the Left Arm Stimulates Excision of Conjugative Transposon Tn916
- Genetic organization of the bacterial conjugative transposon Tn916.
- Sequence analysis of termini of conjugative transposon Tn916.
