DNA-DEPENDENT RNA-DIRECTED PROTEIN SYNTHESIS in vitro, I. EXTENT OF GENOME TRANSCRIPTION*

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RESUMO

We have described an in vitro system which couples UTP incorporation into RNA by DNA-dependent RNA polymerase with RNA-directed amino acid incorporation into polypeptides. Using the technique of DNA-RNA hybridization, we have demonstrated that the whole ΦX-174 genome is transcribed during concomitant protein synthesis.

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