DNA determinants of structural and regulatory variation within the murine beta-glucuronidase gene complex.

AUTOR(ES)

Wawrzyniak, C J

RESUMO

The murine beta-glucuronidase (GUS) gene complex, [Gus], encompasses the GUS structural element, Gus-s, and a set of regulatory elements which serve to modulate Gus-s expression. Three common GUS haplotypes representing virtually all inbred strains of laboratory mice have been compared with respect to GUS mRNA sequence. Results of such comparisons revealed sequence variations which target the location of one of the GUS regulatory elements to sequences within Gus-s and which account for known electrophoretic and heat stability differences among GUS allozymes of the three common GUS haplotypes.

Documentos Relacionados

• Trans-acting temporal locus within the beta-glucuronidase gene complex.
• Genomic organization and sequence of the Gus-s alpha allele of the murine beta-glucuronidase gene.
• Gene fusions of signal sequences with a modified beta-glucuronidase gene results in retention of the beta-glucuronidase protein in the secretory pathway/plasma membrane.
• Detection of active heteropolymeric beta-glucuronidase in hybrids between mouse cells and human fibroblasts with beta-glucuronidase deficiency.
• Correction of murine mucopolysaccharidosis VII by a human beta-glucuronidase transgene.