DNA length, bending, and twisting constraints on IS50 transposition.
AUTOR(ES)
Goryshin IYu
RESUMO
Transposition is a multistep process in which a transposable element DNA sequence moves from its original genetic location to a new site. Early steps in this process include the formation of a transposition complex in which the end sequences of the transposable element are brought together in a structurally precise fashion through the action of the element-encoded transposase protein and the cleavage of the element free from the adjoining DNA. If transposition complex formation must precede DNA cleavage (or nicking), then changing the length of the donor DNA between closely spaced ends should have dramatic effects on the frequency of the transposition. This question has been examined by studying the effects of altering donor DNA length on IS50 transposition. Donor DNA < or = 64 bp severely impaired transposition. Donor DNA > or = 200 bp demonstrated high transposition frequencies with only modest length dependencies. Constructs with donor DNA lengths between 66 and 174 bp demonstrated a dramatic periodic effect on transposition (periodicity approximately 10.5 bp).
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=45120Documentos Relacionados
- Orientation of IS50 transposase gene and IS50 transposition.
- Fis plays a role in Tn5 and IS50 transposition.
- Chromatin remodeling by DNA bending, not twisting
- Integration host factor plays a role in IS50 and Tn5 transposition.
- Identification of base pairs in the outside end of insertion sequence IS50 that are needed for IS50 and Tn5 transposition.
