DNA Polymerase I-Dependent Mutants of Coliphage Lambda
AUTOR(ES)
Shizuya, Hiroaki
RESUMO
Mutagenized lysates of bacteriophage lambda were screened for mutants unable to plate on DNA polymerase I-deficient (polA−) hosts. The mutants obtained were all recombination deficient (red−). These mutants, like red− and gam− mutants previously isolated by others, grow more poorly than wild-type λ even on polA+ hosts (burst size 14 to 30% of wild-type λ.) In a polA− host, the burst size of red− and gam− mutants is reduced an additional five- to tenfold, and lysis is delayed. Wild-type λ grows normally in polA− hosts. Neither λN−nin (which doesn't express red or gam) nor λbio phages (from which all or part of the red-gam region is deleted) form plaques on polA− hosts. Apparent revertants, able to plate on polA− hosts, have been selected from both λN−nin and λbio. Those derived from N−nin seem to be N−nin cro− mutants; whereas those coming from λ bio have a new bypass mutation (pas) that lies between genes P and Q.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=355400Documentos Relacionados
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