DNA sequence of the araC regulatory gene from Escherichia coli B/r.
AUTOR(ES)
Miyada, C G
RESUMO
The DNA sequence of the araC regulatory gene from Escherichia coli B/r has been determined by the base-specific chemical cleavage reactions of Maxam and Gilbert. An open reading frame is found which codes for a protein of 292 amino acids. A nonsense mutation, araC5, is shown to result from a G to A transition at nucleotide 429 converting the tryptophan codon TGG to the amber codon TAG. A deletion which does not recombine with any known point mutation in araC, delta(araCO)719, removes all but the last 22 codons of the gene.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=324300Documentos Relacionados
- Novel activation of araC expression and a DNA site required for araC autoregulation in Escherichia coli B/r.
- Direction of Transcription of the Regulatory Gene araC in Escherichia coli B/r
- Mutations in the araC regulatory gene of Escherichia coli B/r that affect repressor and activator functions of AraC protein.
- DNA sequence of the araBAD promoter in Escherichia coli B/r.
- Dominance relationships among mutant alleles of regulatory gene araC in the Escherichia coli B/R L-arabinose operon.