dnaA acts before dnaC in the initiation of DNA replication.

AUTOR(ES)

Kung, F C

RESUMO

We constructed a double mutant of Escherichia coli K-12 carrying dnaA(Ts) and dnaC(Cs) lesions. In this mutant DNA synthesis proeceeds normally at 32 degrees C and initiation is inhibited at both 41 and 20 degrees C. By shifting this culture grown at 32 degrees C to the two restrictive temperatures in different time sequences and assaying protein and DNA synthesis of cells growing at different temperatures, we found that dnaA and dnaC genes work independently with dnaA acting before dnaC. While preparing special strains for this work, we also showed that the order of genes in the neighborhood of dnaA is dnaA-tnaA-phoS-ilv.

Documentos Relacionados

• Initiation of chromosome replication in dnaA and dnaC mutants of Escherichia coli B/r F.
• Coliphage 186 infection requires host initiation functions dnaA and dnaC.
• Temporal sequence of events during the initiation process in Escherichia coli deoxyribonucleic acid replication: roles of the dnaA and dnaC gene products and ribonucleic acid polymerase.
• Purified dnaA protein in initiation of replication at the Escherichia coli chromosomal origin of replication.
• The DnaA box R4 in the minimal oriC is dispensable for initiation of Escherichia coli chromosome replication.