DNase I sensitivity of integrated simian virus 40 DNA.
AUTOR(ES)
Blanck, G
RESUMO
We undertook an analysis of integrated simian virus 40 (SV40) DNA to learn whether the DNase I-sensitive region is retained in the integrated array of mouse transformants. Our results indicate that full-length integrated SV40 chromatin retains a DNase I-hypersensitive region at the same point as in nonintegrated SV40 chromatin. Thus, the lack of a DNase I-hypersensitive region is not likely to be the reason for nonpermissivity of SV40 in mouse cells. In addition, results reported here indicate that a deletion of about 200 base pairs of DNA in the region of the DNase I-hypersensitive site severely reduces the sensitivity of integrated SV40 chromatin. This result is similar to a previously reported result obtained with deletion mutants of SV40 analyzed in the lytic cycle. It is the first report of a DNA lesion affecting DNase I hypersensitivity of a mammalian chromosome.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=368736Documentos Relacionados
- Location of histones on simian virus 40 DNA.
- Thermal unwinding of simian virus 40 transcription complex DNA.
- BK virus DNA sequence: extent of homology with simian virus 40 DNA.
- Altered restriction endonuclease cleavage pattern of simian virus 40 DNA.
- Structure and formation of circular dimers of simian virus 40 DNA.
