Dnmt3a binds deacetylases and is recruited by a sequence-specific repressor to silence transcription
AUTOR(ES)
Fuks, François
FONTE
Oxford University Press
RESUMO
The Dnmt3a DNA methyltransferase is essential for mammalian development and is responsible for the generation of genomic methylation patterns, which lead to transcriptional silencing. Here, we show that Dnmt3a associates with RP58, a DNA-binding transcriptional repressor protein found at transcriptionally silent heterochromatin. Dnmt3a acts as a co-repressor for RP58 in a manner that does not require its de novo methyltransferase activity. Like other characterized co-repressors, Dnmt3a associates with the histone deacetylase HDAC1 using its ATRX-homology domain. This domain of Dnmt3a represents an independent transcriptional repressor domain whose silencing functions require HDAC activity. These results identify Dnmt3a as a co-repressor protein carrying deacetylase activity and show that Dnmt3a can be targeted to specific regulatory foci via its association with DNA-binding transcription factors.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=125250Documentos Relacionados
- Brinker is a sequence-specific transcriptional repressor in the Drosophila embryo
- BCL-6, a POZ/zinc-finger protein, is a sequence-specific transcriptional repressor.
- Adenovirus type 2 VAI RNA transcription by polymerase III is blocked by sequence-specific methylation.
- DEC1 is a downstream target of TGF-β with sequence-specific transcriptional repressor activities
- Sequence-specific Rho–RNA interactions in transcription termination