Dose Response Curve Linearization and Computer Potency Calculation of Turbidimetric Microbiological Vitamin Assays

AUTOR(ES)

Tsuji, Kiyochi

RESUMO

The dose response curves of various turbidimetric microbiological assays, including vitamin B12, Ca pantothenate, and pyridoxine (vitamin B6), were linear with logarithmic transformation of the responses by use of the equation derived, ln[T(x) - T(∞)] = ln A - Bx. A Fortran computer program which used the slope ratio potency calculation was written. The assay potencies calculated by the computer program showed excellent agreement with those obtained by the manual calculation.

Documentos Relacionados

• Turbidimetric Assays: the Antibiotic Dose-Response Line
• Semiautomated Method for Microbiological Vitamin Assays
• Semiautomated Turbidimetric Microbiological Assay for Determination of Cefazolin
• THE CALCULATION OF MICROBIAL ASSAYS
• Comparison of pharmacopeial statistical methods applied in microbiological assay for antibiotics potency determination using parallel lines and three-dose level