Double-Osmotic-Shock Procedure to Prepare Gentamicin Adenine Mononucleotide Transferase for the Enzymatic Assay of Gentamicin
AUTOR(ES)
Dankert, J.
RESUMO
The release of gentamicin adenine mononucleotide transferase during single, cold, osmotic-shock treatment of Escherichia coli K-12 W677/HJR66 is not always maximal. The quantity of gentamicin adenine mononucleotide transferase in osmotic extracts could be significantly increased when a double-shock procedure was performed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=352133Documentos Relacionados
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