Double-Staining Method for Differentiation of Morphological Changes and Membrane Integrity of Campylobacter coli Cells
AUTOR(ES)
Alonso, Jose L.
FONTE
American Society for Microbiology
RESUMO
We developed a double-staining procedure involving NanoOrange dye (Molecular Probes, Eugene, Oreg.) and membrane integrity stains (LIVE/DEAD BacLight kit; Molecular Probes) to show the morphological and membrane integrity changes of Campylobacter coli cells during growth. The conversion from a spiral to a coccoid morphology via intermediary forms and the membrane integrity changes of the C. coli cells can be detected with the double-staining procedure. Our data indicate that young or actively growing cells are mainly spiral shaped (green-stained cells), but older cells undergo a degenerative change to coccoid forms (red-stained cells). Club-shaped transition cell forms were observed with NanoOrange stain. Chlorinated drinking water affected the viability but not the morphology of C. coli cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=126449Documentos Relacionados
- Fluorescent treponemal antibody-absorption double-staining procedure.
- Fluorescent Treponemal Antibody Absorption Double-Staining Test Evaluation
- Double-staining procedure for the fluorescent treponemal antibody absorption (FTA-ABS) test.
- Further studies with the fluorescent treponemal antibody-absorption double-staining procedure.
- Detection of erythrocyte membrane proteins, sialoglycoproteins, and lipids in the same polyacrylamide gel using a double-staining technique.
