Drosophila transcriptional repressor protein that binds specifically to negative control elements in fat body enhancers.
AUTOR(ES)
Falb, D
RESUMO
Expression of the Drosophila melanogaster Adh gene in adults requires a fat body-specific enhancer called the Adh adult enhancer (AAE). We have identified a protein in Drosophila nuclear extracts that binds specifically to a site within the AAE (adult enhancer factor 1 [AEF-1]). In addition, we have shown that AEF-1 binds specifically to two other Drosophila fat body enhancers. Base substitutions in the AEF-1 binding site that disrupt AEF-1 binding in vitro result in a significant increase in the level of Adh expression in vivo. Thus, the AEF-1 binding site is a negative regulatory element within the AAE. A cDNA encoding the AEF-1 protein was isolated and shown to act as a repressor of the AAE in cotransfection studies. The AEF-1 protein contains four zinc fingers and an alanine-rich sequence. The latter motif is found in other eukaryotic proteins known to be transcriptional repressors.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=360306Documentos Relacionados
- Characterization of a protein that binds multiple sequences in mammalian type C retrovirus enhancers.
- Helix-loop-helix transcriptional activators bind to a sequence in glucocorticoid response elements of retrovirus enhancers.
- Purification of core-binding factor, a protein that binds the conserved core site in murine leukemia virus enhancers.
- Redundant cis-acting elements control expression of the Drosophila affinidisjuncta Adh gene in the larval fat body.
- Two factors that bind to highly conserved sequences in mammalian type C retroviral enhancers.