Dual Regulatory Control of a Particle Maturation Function of Bacteriophage P1

AUTOR(ES)

Lehnherr, Hansjörg

FONTE

American Society for Microbiology

RESUMO

A unique arrangement of promoter elements was found upstream of the bacteriophage P1 particle maturation gene (mat). A P1-specific late-promoter sequence with conserved elements located at positions −22 and −10 was expected from the function of the gene in phage morphogenesis. In addition to a late-promoter sequence, a −35 element and an operator sequence for the major repressor protein, C1, were found. The −35 and −10 elements constituted an active Escherichia coli ς70 consensus promoter, which was converted into a P1-regulated early promoter by the superimposition of a C1 operator. This combination of early- and late-promoter elements regulates and fine-tunes the expression of the particle maturation gene. During lysogenic growth the gene is turned off by P1 immunity functions. Upon induction of lytic growth, the expression of mat starts simultaneously with the expression of other C1-regulated P1 early functions. However, while most of the latter functions are downregulated during late stages of lytic growth the expression of mat continues throughout the entire lytic growth cycle of bacteriophage P1. Thus, the maturation function has a head start on the structural components of the phage particle.

Documentos Relacionados

• Stimulation of IS1 excision by bacteriophage P1 ref function.
• Maintenance of bacteriophage P1 plasmid.
• Curing of P1 prophage from Escherichia coli K-12 recA(P1) lysogens superinfected with P1 bacteriophage.
• Enhancement of Escherichia Coli Plasmid and Chromosomal Recombination by the Ref Function of Bacteriophage P1
• DRUG RESISTANCE OF ENTERIC BACTERIA IV. : Active Transducing Bacteriophage P1 CM Produced by the Combination of R Factor With Bacteriophage P1