Dynamic methylation of histone H3 at lysine 4 in transcriptional regulation by the androgen receptor
AUTOR(ES)
Kim, Joshua
FONTE
Oxford University Press
RESUMO
The methylation of histone H3 correlates with either gene expression or silencing depending on the residues modified. Methylated lysine 4 (H3-K4) is associated with transcription at active gene loci. Furthermore, it was reported that trimethylated but not dimethylated H3-K4 is exclusively associated with active chromatin in Saccharomyces cerevisiae. In the present study, we investigated the H3-K4 methylation at the human prostate specific antigen (PSA) locus following gene activation and repression via androgen receptor (AR). We show that ligand-induced, AR-mediated transcription was accompanied by rapid decreases in di- and trimethylated H3-K4 at the PSA enhancer and promoter. Moreover, the observed decreases in H3-K4 methylation were reversed when AR was inhibited by a specific AR antagonist, bicalutamide. In contrast to the decreases in methylation at the 5′ transcriptional control regions of the PSA gene, H3-K4 methylation in the coding region steadily increased after a lag period of ∼4 h. The results suggest a novel role of methylated H3-K4 in transcriptional regulation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=290276Documentos Relacionados
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