Early gene expression in bacteriophage T7. I. In vivo synthesis, inactivation, and translational utilization of early mRNA's.
AUTOR(ES)
Hercules, K
RESUMO
In vivo decay rates for the individual T7 early mRNA species were determined. The physical half-lives, measured at 37 C, range from 1.1 min for gene 0.7 RNA to 4.5 min for gene 0.3 RNA. Physical half-lives, as observed after rifampin inhibition of RNA synthesis and polyacylamide electrophoresis of RNAs, are approximately 30% longer than functional half-lives, as observed by 14C-labeled amino acid uptake into individual T7 early proteins. The different RNA species are synthesized at grossly different rates, 0.3 RNA at four times the rate of 1.0 RNA, 0.7 RNA at twice the rate, and 1.1 and 1.3 RNAs at about the same or a slightly lower rate than 1.0 RNA. Rho-factor-mediated termination of transcription behind genes 0.3, 0.7, and perhaps behind 1.0 is inferred from these data. The in vivo translational utilization of the individual T7 early-message species was found to vary by not more than a factor of 2.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=515454Documentos Relacionados
- Chemical stability of bacteriophage T7 early mRNA.
- Simian virus 40 early mRNA's. I. Genomic localization of 3' and 5' termini and two major splices in mRNA from transformed and lytically infected cells.
- Regulation of Early mRNA Synthesis in Bacteriophage T4-Infected Bacteria: Dependence on Bacteriophage-Specific Protein Synthesis
- Regulation of Early mRNA Synthesis in Bacteriophage T4-Infected Bacteria: Dependence on Bacteriophage-Specific Protein Synthesis
- Synthesis of functional bacteriophage T4-delayed early mRNA in the absence of protein synthesis.